Table 2 Associations of true methylation and hydroxymethylation of NPPA promoter with the functional outcome at 3-month follow up after ischemic stroke onset
CpG Loci | Genomic Position, GRCh37 | Relative to TSS, bp | Level, % | OR*(95%CI) | P value | q value |
|---|---|---|---|---|---|---|
True methylation | ||||||
CpG1 | Chr1:11908353 | -513 | 25.31 ± 5.21 | 0.37(0.22–0.62) | 1.48E-04 | 4.69E-04 |
CpG2 | Chr1:11908348 | -508 | 91.19 ± 3.52 | 0.17(0.01–2.06) | 1.60E-01 | 1.60E-01 |
CpG3 | Chr1:11908299 | -459 | 19.66 ± 4.39 | 0.56(0.37–0.84) | 5.87E-03 | 8.80E-03 |
CpG4 | Chr1:11908200 | -360 | 66.03 ± 7.17 | 0.19(0.08–0.47) | 3.54E-04 | 7.97E-04 |
CpG5 | Chr1:11908182 | -342 | 78.93 ± 5.75 | 0.17(0.04–0.62) | 7.37E-03 | 9.47E-03 |
CpG6 | Chr1:11908178 | -338 | 37.55 ± 6.37 | 0.30(0.16–0.54) | 7.68E-05 | 4.69E-04 |
CpG7 | Chr1:11908168 | -328 | 48.01 ± 7.03 | 0.35(0.18–0.70) | 3.03E-03 | 5.46E-03 |
CpG8 | Chr1:11908165 | -325 | 28.44 ± 6.79 | 0.43(0.28–0.67) | 1.56E-04 | 4.69E-04 |
CpG9 | Chr1:11908142 | -302 | 35.81 ± 8.56 | 0.61(0.40–0.93) | 2.08E-02 | 2.33E-02 |
Hydroxymethylation | ||||||
CpG1 | Chr1:11908353 | -513 | 1.02 ± 1.59 | 0.94(0.75–1.17) | 5.72E-01 | 8.17E-01 |
CpG2 | Chr1:11908348 | -508 | 1.52 ± 1.71 | 0.93(0.77–1.12) | 4.45E-01 | 8.17E-01 |
CpG3 | Chr1:11908299 | -459 | 2.50 ± 2.41 | 1.11(0.95–1.31) | 1.82E-01 | 8.17E-01 |
CpG4 | Chr1:11908200 | -360 | 1.14 ± 1.23 | 1.12(0.89–1.41) | 3.46E-01 | 8.17E-01 |
CpG5 | Chr1:11908182 | -342 | 1.88 ± 1.76 | 1.05(0.86–1.27) | 6.41E-01 | 8.17E-01 |
CpG6 | Chr1:11908178 | -338 | 0.86 ± 1.10 | 0.94(0.73–1.20) | 6.06E-01 | 8.17E-01 |
CpG7 | Chr1:11908168 | -328 | 1.05 ± 1.38 | 1.04(0.82–1.32) | 7.26E-01 | 8.17E-01 |
CpG8 | Chr1:11908165 | -325 | 0.86 ± 1.09 | 1.00(0.78–1.30) | 9.79E-01 | 9.79E-01 |
CpG9 | Chr1:11908142 | -302 | 0.77 ± 0.91 | 0.92(0.70–1.20) | 5.47E-01 | 8.17E-01 |