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Fig. 4 | Epigenetics Communications

Fig. 4

From: qBiCo: a method to assess global DNA conversion performance in epigenetics via single-copy genes and repetitive elements

Fig. 4

Comparative qBiCo-v2 assay performance on six qPCR instruments. (A) PCR efficiency (%) and (B) goodness-of-fit values (R2), of each qPCR assay of qBiCo-v2. Data are included from the initial method transfer runs on six qPCR systems from three biotechnology manufacturers (BioRad, Thermo Fisher Scientific, Qiagen). Dashed red lines indicate ideal performance measurements, while the grey box indicates acceptable range by standard qPCR guidelines (PCR efficiency between 90–110%)

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