Fig. 5

qBiCo-v2 assay validation using synthetic DNA standards on six qPCR instruments. Testing (A-C) repeatability at two DNA inputs, (D-F) sensitivity down to 0.02 ng/µl, and (G-I) robustness at different UV exposure times, per qBiCo index and qPCR instrument (colour-coded). All qBiCo indices are presented in ratios. BC: Bisulfite conversion