Table 1 Information on bisulfite conversion (BC) kits used during the testing of qBiCo-v1 method
Kit No | Manufacturer | Kit name | Incubation protocol |
|---|---|---|---|
1 | Zymo Research | EZ DNA Methylation | 14–16 h at 50 °C |
2 | Thermo Fisher Scientific | EpiJET BC | 10 min at 98 °C / 150 min at 60 °C |
3 | Diagenode | Premium Bisulfite | 8 min at 98 °C / 60 min at 54 °C |
4 | Promega | MethylEdge® BC System | 8 min at 98 °C / 60 min at 54 °C |
5 | Sigma Aldrich | Imprint® DNA Modification | 90 min at 65 °C |
6 | Qiagen | EpiTect Bisulfite | 5 min at 95 °C / 25 min at 60 °C / 5 min at 95 °C / 85 min at 60 °C / 5 min at 95 °C / 175 min at 60 °C |
7 | Abcam | Fast BC | 20 min at 95 °C |
8 | Analytik Jena | innuCONVERT | 45 min at 85 °C |
9 | Epigentek | Methylamp DNA modification | 90 min at 65 °C |
10 | Active Motif | BC | 30 s at 95 °C / 20 min at 58 °C |