Table 2 Composition of the updated synthetic DNA standard (STD) in qBiCo-v2 method
qPCR assay | Number of copies (DNA molecules) | ||||
|---|---|---|---|---|---|
StdA | StdB | StdC | StdD | StdE | |
hTERT Short | 6,000 | 2,000 | 667 | 222 | 74 |
TPT1 Long | 6,000 | 2,000 | 667 | 222 | 74 |
LINE1 Converted | 384,000 | 128,000 | 42,667 | 14,222 | 4,741 |
LINE1 Genomic | 11,400 | 3,800 | 1,267 | 422 | 141 |
Synthetic IPC | 3,000 | 3,000 | 3,000 | 3,000 | 3,000 |